Overall Categorization
Attributes of FASTQ
Alphabet Frequency & GC content
eel_PE1300
Attributes of FASTQ
Name Value InputFile eel_PE1300.fastq #Read 1,000,000 #Base 96,230,170 AvgReadLen 96.23 MinReadLen 35 MaxReadLen 101
Alphabet Frequency & GC content
Name Count Freq% A 28,107,801 29.21% C 20,190,282 20.98% G 19,660,165 20.43% T 28,189,280 29.29% N 82,642 0.09% GC% - 41.41%
Distribution of Bases' Quality Values
Name AreaFreq Q30 & above 88.0% Q20-Q29 8.8% Q15-Q19 1.5% < Q15 1.8%
Distribution of Reads' MinimalQ Values
Name AreaFreq % of reads whose bases are all Q20 & above 51.4% % of reads whose bases are all Q15 & above 61.4% % of reads whose bases are all Q10 & above 68.6%
Coverage of Reads with Sufficient High-Quality Bases
Name Coverage of reads Remark % of High-quality reads
Coverage of reads that 100% of their bases with Q20 & above 51.4% %HighQ(20) >= 100%
(i.e., MinimaQ>=20) Coverage of reads that >= 95% of their bases with Q20 & above 78.5% %HighQ(20) >= 95% Coverage of reads that >= 90% of their bases with Q20 & above 87.1% %HighQ(20) >= 90% Coverage of reads that >= 90% of their bases with Q15 & above 93.4% %HighQ(15) >= 90% % of Poor-quality reads
Coverage of reads that > 10% of their bases with Q14 & less 6.6% 1 - {%HighQ(15) >= 90%}
Attributes of FASTQ
| Name | Value |
|---|---|
| InputFile | eel_PE1300.fastq |
| #Read | 1,000,000 |
| #Base | 96,230,170 |
| AvgReadLen | 96.23 |
| MinReadLen | 35 |
| MaxReadLen | 101 |
Alphabet Frequency & GC content
| Name | Count | Freq% |
|---|---|---|
| A | 28,107,801 | 29.21% |
| C | 20,190,282 | 20.98% |
| G | 19,660,165 | 20.43% |
| T | 28,189,280 | 29.29% |
| N | 82,642 | 0.09% |
| GC% | - | 41.41% |
Distribution of Bases' Quality Values
| Name | AreaFreq |
|---|---|
| Q30 & above | 88.0% |
| Q20-Q29 | 8.8% |
| Q15-Q19 | 1.5% |
| < Q15 | 1.8% |
Distribution of Reads' MinimalQ Values
| Name | AreaFreq |
|---|---|
| % of reads whose bases are all Q20 & above | 51.4% |
| % of reads whose bases are all Q15 & above | 61.4% |
| % of reads whose bases are all Q10 & above | 68.6% |
Coverage of Reads with Sufficient High-Quality Bases
| Name | Coverage of reads | Remark |
|---|---|---|
| % of High-quality reads Coverage of reads that 100% of their bases with Q20 & above | 51.4% | %HighQ(20) >= 100% (i.e., MinimaQ>=20) |
| Coverage of reads that >= 95% of their bases with Q20 & above | 78.5% | %HighQ(20) >= 95% |
| Coverage of reads that >= 90% of their bases with Q20 & above | 87.1% | %HighQ(20) >= 90% |
| Coverage of reads that >= 90% of their bases with Q15 & above | 93.4% | %HighQ(15) >= 90% |
| % of Poor-quality reads Coverage of reads that > 10% of their bases with Q14 & less | 6.6% | 1 - {%HighQ(15) >= 90%} |
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